Purification Scientist
Boston, MA
Experience: 0
Category: Scientific & Clinical
Contractor Work Model: Onsite
Brand: Joulé
Compensation Range: 39.60 - 45.40 Per Hour
Job ID: 346385
Date Posted: 02/20/2026
Shortcut: http://jobs.systemone.com/dEyDEm
Job Title: Purification Scientist – Downstream Process Development
Location: Boston, MA
Hours/Schedule: Monday–Friday, 8 am to 5 pm
Compensation: $40.00 - $45.40/hr
Type: 12 month contract, Potential for extension or conversion to permanent
Overview
The Purification Scientist supports downstream process development for viral vectors, biologics, and recombinant proteins. This role focuses on executing and optimizing purification workflows, generating high-quality data, and supporting manufacturing readiness under GMP/GLP-aligned practices. The ideal candidate brings strong hands-on chromatography and ultracentrifugation experience, with the ability to troubleshoot processes and collaborate across technical teams.
Responsibilities
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Execute purification workflows including CsCl density gradient ultracentrifugation, affinity chromatography (e.g., AVB, CaptureSelect), ion-exchange chromatography (AEX, CEX), and polishing steps
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Operate AKTA systems (Avant, Pure, or Pilot) to perform small- to large-scale purification runs
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Perform tangential flow filtration (TFF) for concentration and buffer exchange of intermediates and final products
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Design and execute experiments to support process optimization and development initiatives
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Collect, analyze, and document data in accordance with established procedures and GMP/GLP best practices
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Perform analytical characterization using methods such as UV spectroscopy, SDS-PAGE, ddPCR, ELISA, and HPLC
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Troubleshoot purification challenges and recommend process improvements
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Collaborate cross-functionally with upstream development, analytical, and manufacturing teams
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Maintain laboratory equipment, prepare buffers, manage chromatography columns, and ensure compliance with safety and documentation standards
Qualifications
Required:
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Master’s degree in Biology, Biochemistry, Chemical Engineering, Biotechnology, or related field
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Minimum 2 years of hands-on purification experience in academic or industry settings
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Practical experience with CsCl density gradient ultracentrifugation, ion-exchange chromatography, and affinity purification
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Experience with buffer preparation and purification process fundamentals
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Understanding of analytical methods for biologic or viral vector characterization
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Strong documentation practices and attention to detail
Preferred:
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Experience with AAV or other viral vector purification using CsCl density gradients and column chromatography
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Experience operating AKTA systems and Unicorn software
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Familiarity with regulated laboratory environments supporting GMP or GLP activities
Benefits
System One offers eligible employees health and welfare benefits coverage options, including medical, dental, vision, spending accounts, life insurance, voluntary plans, and participation in a 401(k) plan.
Ref: #558-Scientific
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